{"id":422357,"date":"2024-09-02T16:42:03","date_gmt":"2024-09-02T08:42:03","guid":{"rendered":"https:\/\/www.youtupa.com\/jiaotong\/422357.html"},"modified":"2024-09-02T23:18:53","modified_gmt":"2024-09-02T15:18:53","slug":"%e5%8f%af%e6%8c%81%e7%bb%ad%e6%80%a7%e8%ae%be%e8%ae%a1%e5%8e%9f%e5%88%99-%e5%8f%af%e6%8c%81%e7%bb%ad%e6%80%a7%e8%ae%be%e8%ae%a1%e5%8e%9f%e5%88%99%e6%98%af%e4%bb%80%e4%b9%88","status":"publish","type":"post","link":"https:\/\/www.youtupa.com\/jiaotong\/422357.html","title":{"rendered":"\u53ef\u6301\u7eed\u6027\u8bbe\u8ba1\u539f\u5219 \u53ef\u6301\u7eed\u6027\u8bbe\u8ba1\u539f\u5219\u662f\u4ec0\u4e48"},"content":{"rendered":"<figure class=\"wp-block-image size-full aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"300\" src=\"https:\/\/www.youtupa.com\/wp-content\/uploads\/2024\/09\/d57ec863e48291e920d77356387f16f4.png\" class=\"attachment-post-thumbnail size-post-thumbnail wp-post-image\" alt=\"\u53ef\u6301\u7eed\u6027\u8bbe\u8ba1\u539f\u5219 \u53ef\u6301\u7eed\u6027\u8bbe\u8ba1\u539f\u5219\u662f\u4ec0\u4e48\" title=\"\u53ef\u6301\u7eed\u6027\u8bbe\u8ba1\u539f\u5219 \u53ef\u6301\u7eed\u6027\u8bbe\u8ba1\u539f\u5219\u662f\u4ec0\u4e48\u7f29\u7565\u56fe\" \/><\/figure>\n<p> \u4e00\u3001webquest\u8bbe\u8ba1\u539f\u5219\uff1fwebquest\u6630\u4e00\u79cd\u57fa\u4e8e\u7f51\u7edc\u7684\u3001\u4efb\u52a1\u9a71\u52a8\u5f0f\u7684\u5b66\u4e60\u6a21\u5f0f\uff0c\u540c\u65f6\u4e5f\u662f\u7f51\u7edc\u4e0a\u6700\u9002\u5408\u9f13\u52b1\u9ad8\u7ea7\u601d\u7ef4\u6280\u5de7\u548c\u5f3a\u8c03\u4ee5\u771f\u5b9e\u7ea6\u65b9\u5f0f\u5229\u7528\u4fe1\u606f\u7684\u5b66\u4e60\u6a21\u5f0f\u3002\u5176\u8bbe\u8ba1\u539f\u5219\u5305\u62ec\u4ee5\u4e0b5 <\/p>\n<h2>\u4e00\u3001webquest\u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>webquest\u6630\u4e00\u79cd\u57fa\u4e8e\u7f51\u7edc\u7684\u3001\u4efb\u52a1\u9a71\u52a8\u5f0f\u7684\u5b66\u4e60\u6a21\u5f0f\uff0c\u540c\u65f6\u4e5f\u662f\u7f51\u7edc\u4e0a\u6700\u9002\u5408\u9f13\u52b1\u9ad8\u7ea7\u601d\u7ef4\u6280\u5de7\u548c\u5f3a\u8c03\u4ee5\u771f\u5b9e\u7ea6\u65b9\u5f0f\u5229\u7528\u4fe1\u606f\u7684\u5b66\u4e60\u6a21\u5f0f\u3002\u5176\u8bbe\u8ba1\u539f\u5219\u5305\u62ec\u4ee5\u4e0b5\u4e2a\u65b9\u9762\uff0c\u5373\uff1a<\/p>\n<p>1\u3001\u627e\u51fa\u7cbe\u5f69\u7684\u7f51\u7ad9\uff1b<\/p>\n<p>2\u3001\u6709\u6548\u5730\u7ec4\u7ec7\u5b66\u751f\u548c\u5b66\u4e60\u8d44\u6e90\uff1b<\/p>\n<p>3\u3001\u8981\u6c42\u5b66\u751f\u601d\u8003\uff1b<\/p>\n<p>4\u3001\u9009\u7528\u5a92\u4f53\uff1b<\/p>\n<p>5\u3001\u4e3a\u9ad8\u6c34\u5e73\u7684\u5b66\u4e60\u671f\u671b\u642d\u5efa\u811a\u624b\u67b6\u3002<\/p>\n<h2>\u4e8c\u3001\u8863\u67dc\u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>\u5916\u8868\u7f8e\u89c2\uff0c\u5185\u90e8\u6536\u7eb3\u8863\u7269\u65b9\u4fbf\uff0c\u5360\u7528\u5730\u65b9\u9002\u4e2d\uff0c\u9ad8\u77ee\u9002\u5b9c<\/p>\n<h2>\u4e09\u3001\u6807\u5fd7\u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>1\u3001\u6784\u601d\u6df1\u523b\u3001\u6784\u56fe\u7b80\u6d01\uff1b<\/p>\n<p>2\u3001\u65b0\u9896\u522b\u81f4\u3001\u72ec\u5177\u4e00\u683c\uff1b<\/p>\n<p>3\u3001\u5f62\u8c61\u751f\u52a8\u3001\u6613\u4e8e\u8bc6\u522b\u3002<\/p>\n<h2>\u56db\u3001antdesign\u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>\u4e00\uff0c\u4eb2\u5bc6\u6027\u3002\u5982\u679c\u4fe1\u606f\u4e4b\u95f4\u5173\u8054\u6027\u8d8a\u63a5\u8fd1\uff0c\u4e5f\u8d8a\u50cf\u4e00\u4e2a\u89c6\u89c9\u5355\u5143\u3002\u53cd\u4e4b\uff0c\u4ed6\u4eec\u7684\u8ddd\u79bb\u5c31\u5e94\u8be5\u8d8a\u8fdc\uff0c\u8d8a\u50cf\u591a\u4e2a\u89c6\u89c9\u5355\u5143\u3002\u4eb2\u5bc6\u6027\u7684\u6839\u672c\u76ee\u7684\u662f\u5b9e\u73b0\u7ec4\u7ec7\u6027\uff0c\u8ba9\u7528\u6237\u5bf9\u9875\u9762\u7ed3\u6784\u548c\u4fe1\u606f\u5c42\u6b21\u4e00\u76ee\u4e86\u7136\u3002<\/p>\n<p>\u4e8c\uff0c\u5bf9\u9f50\u3002\u5728\u77e5\u89c9\u8fc7\u7a0b\u4e2d\u4eba\u4eec\u5f80\u5f80\u503e\u5411\u4e8e\u662f\u77e5\u89c9\u5bf9\u8c61\u7684\u76f4\u7ebf\u7ee7\u7eed\u6210\u4e3a\u76f4\u7ebf\uff0c\u4f7f\u66f2\u7ebf\u7ee7\u7eed\u6210\u4e3a\u66f2\u7ebf\u3002\u5728\u754c\u9762\u8bbe\u8ba1\u4e2d\uff0c\u5c06\u5143\u7d20\u8fdb\u884c\u5bf9\u9f50\uff0c\u65e2\u7b26\u5408\u7528\u6237\u7684\u8ba4\u77e5\u7279\u6027\uff0c\u4e5f\u80fd\u5f15\u5bfc\u89c6\u89c9\u6d41\u5411\uff0c\u8ba9\u7528\u6237\u66f4\u52a0\u6d41\u7545\u7684\u63a5\u6536\u4fe1\u606f\u3002<\/p>\n<p>\u4e09\uff0c\u5bf9\u6bd4\u3002\u5bf9\u6bd4\u662f\u589e\u52a0\u89c6\u89c9\u6548\u679c\u6700\u6709\u6548\u65b9\u6cd5\u4e4b\u4e00\uff0c\u540c\u65f6\uff0c\u80fd\u5728\u4e0d\u540c\u5143\u7d20\u4e4b\u95f4\u5efa\u7acb\u4e00\u79cd\u6709\u7ec4\u7ec7\u7684\u5c42\u6b21\u7ed3\u6784\uff0c\u8ba9\u7528\u6237\u5feb\u901f\u8bc6\u522b\u5173\u952e\u4fe1\u606f\u3002<\/p>\n<p>\u56db\uff0c\u91cd\u590d\u3002\u76f8\u540c\u7684\u5143\u7d20\u5728\u6574\u4e2a\u754c\u9762\u4e2d\u4e0d\u65ad\u91cd\u590d\uff0c\u4e0d\u4ec5\u53ef\u4ee5\u6709\u6548\u964d\u4f4e\u7528\u6237\u7684\u5b66\u4e60\u6210\u672c\uff0c\u4e5f\u53ef\u4ee5\u5e2e\u52a9\u7528\u6237\u8bc6\u522b\u51fa\u8fd9\u4e9b\u5143\u7d20\u4e4b\u95f4\u7684\u5173\u8054\u6027\u3002<\/p>\n<p>\u4e94\uff0c\u76f4\u622a\u4e86\u5f53\u3002\u9700\u8981\u5728\u54ea\u91cc\u8f93\u51fa\uff0c\u5c31\u662f\u5141\u8bb8\u5728\u54ea\u91cc\u8f93\u5165\uff0c\u8fd9\u5c31\u662f\u76f4\u63a5\u64cd\u4f5c\u7684\u539f\u7406\u3002<\/p>\n<p>\u516d\uff0c\u8db3\u4e0d\u51fa\u6237\u3002\u80fd\u5728\u8fd9\u4e2a\u9875\u9762\u89e3\u51b3\u7684\u95ee\u9898\uff0c\u5c31\u4e0d\u8981\u53bb\u5176\u4ed6\u9875\u9762\u89e3\u51b3\u3002<\/p>\n<p>\u4e03\uff0c\u7b80\u5316\u4ea4\u4e92\u3002<\/p>\n<p>\u516b\uff0c\u63d0\u4f9b\u9080\u8bf7\u3002<\/p>\n<p>\u4e5d\uff0c\u5de7\u7528\u8fc7\u6e21\u3002<\/p>\n<p>\u5341\uff0c\u5373\u65f6\u53cd\u5e94\u3002<\/p>\n<h2>\u4e94\u3001grna\u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>\u8bbe\u8ba1gRNA\uff0c\u9700\u8981\u5728\u57fa\u56e0\u7ec4\u4e2d\u627e\u5230\u9002\u5f53\u7684\u76ee\u6807\u5e8f\u5217\u3002<\/p>\n<p>\u57fa\u56e0\u7ec4\u91cc\u7684\u9776\u70b9\uff0c\u8981\u6db5\u76d6\u4ee5\u4e0b\u7279\u5f81\uff1a<\/p>\n<p>\u957f\u5ea6\u5927\u7ea6\u572820\u4e2a\u6838\u82f7\u9178\u3002<\/p>\n<p>\u540e\u9762\uff08\u6216\u8005\u524d\u9762\uff09\u662fPAM\u5e8f\u5217\u3002PAM\u5e8f\u5217\u4f1a\u968fCas9\u6765\u6e90\u7684\u83cc\u79cd\u4e0d\u540c\u800c\u53d8\u5316\uff0cgRNA\u9700\u8981\u4e0e\u83cc\u79cd\u4e00\u81f4\u3002<\/p>\n<p>\u8bbe\u8ba1\u7684gRNA\uff0c\u8981\u6c42\uff1a<\/p>\n<p>\u4e0d\u5305\u542bPAM\u5e8f\u5217\u3002<\/p>\n<p>\u53ef\u4ee5\u662f\u57fa\u56e0\u7ec4DNA\u7684\u4efb\u610f\u4e00\u6761\u94fe\u3002<\/p>\n<p>\u5982\u679c\u8981Cas9\u7834\u574f\u57fa\u56e0\u7ec4\uff0cgRNA\u9776\u70b9\u5e94\u8be5\u9760\u8fd1\u86cb\u767d\u7f16\u7801N\u7aef\uff0c\u4ee5\u4fbf\u589e\u52a0\u57fa\u56e0\u7834\u574f\u7684\u53ef\u80fd\u6027\u3002<\/p>\n<p>\u5982\u679c\u8981Cas9\u5bf9\u57fa\u56e0\u7ec4\u4f5c\u7f16\u8f91\u6216\u8005\u4fee\u9970\uff0cgRNA\u9776\u70b9\u5c06\u9650\u4e8e\u7f16\u8f91\u6216\u8005\u4fee\u9970\u7684\u4f4d\u7f6e\u3002<\/p>\n<p>\u9009\u62e9\u751f\u7269\u4fe1\u606f\u5b66\u8f6f\u4ef6\u6765\u8bbe\u8ba1\uff0c\u907f\u514d\u8131\u9776\u73b0\u8c61\u3002<\/p>\n<h2>\u516d\u3001ets\u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>1.<\/p>\n<p>\u91c7\u7528\u6545\u969c\u5b89\u5168\u51c6\u5219\u3002\u5982\u4e27\u5931\u63a7\u5236\u6db2\u538b\u5c06\u7acb\u5373\u5173\u95ed4\u6709\u7684 \u4e3b\u6c7d\u95e8\u53ca\u8c03\u8282\u9600\u3002<\/p>\n<p>2.<\/p>\n<p>\u91c7\u7528\u4e0d\u540c\u539f\u7406\u7684\u505c\u673a\u65b9\u5f0f\u3002\u673a\u68b0\u5f15\u53d1\u7684\u505c\u673a\u548c\u7535\u6c14\u5f15\u53d1\u7684\u505c \u673a,\u589e\u52a0\u4e86\u7cfb\u7edf\u7684\u53ef\u9760\u6027\u3002<\/p>\n<p>3.<\/p>\n<p>\u591a\u91cd\u6027\u3002\u5982\u505c\u673a\u53c2\u6570\u5747\u91c7\u7528\u591a\u901a\u9053,C\u91c7\u7528\u4e09\u5957,1\/0\u5361 \u7b49\u4e5f\u662f\u4e09\u5957,\u4e09\u53d6\u4e8c\u8df3\u673a[\u4e00\u822c\u7684DCS\u63a7\u5236\u7cfb\u7edf,\u4ec5\u6709\u63a7\u5236\u5668(\u5305 \u62ec\u901a\u4fe1\u5361\u7b49)&#8230;<\/p>\n<p>4.<\/p>\n<p>\u5c40\u90e8\u903b\u8f91\u91c7\u7528\u4e09\u53d6\u4e8c\u6216\u56db\u53d6\u4e8c\u7b49,\u589e\u52a0\u53ef\u9760\u6027\u3002<\/p>\n<h2>\u4e03\u3001\u8bbe\u8ba1\u56db\u539f\u5219\uff1f<\/h2>\n<p>\u8981\u60f3\u6210\u4e3a\u4e00\u4e2a\u4f18\u79c0\u7684\u8bbe\u8ba1\u5e08\uff0c\u5c31\u5fc5\u987b\u8981\u77e5\u9053\u8bbe\u8ba1\u7684\u56db\u5927\u57fa\u672c\u539f\u5219\u662f\u4ec0\u4e48\uff1f\u8bbe\u8ba1\u7684\u56db\u5927\u57fa\u672c\u539f\u5219\uff1a\u4eb2\u5bc6\u6027\u3001\u5bf9\u9f50\u3001\u5bf9\u6bd4\u3001\u91cd\u590d\u3002<\/p>\n<p>\u8bbe\u8ba1\u662f\u628a\u4e00\u79cd\u8bbe\u60f3\u901a\u8fc7\u5408\u7406\u7684\u89c4\u5212\u3001\u5468\u5bc6\u7684\u8ba1\u5212\u3001\u901a\u8fc7\u5404\u79cd\u65b9\u5f0f\u8868\u8fbe\u51fa\u6765\u7684\u8fc7\u7a0b\u3002\u4eba\u7c7b\u901a\u8fc7\u52b3\u52a8\u6539\u9020\u4e16\u754c\uff0c\u521b\u9020\u6587\u660e\uff0c\u521b\u9020\u7269\u8d28\u8d22\u5bcc\u548c\u7cbe\u795e\u8d22\u5bcc\uff0c\u800c\u6700\u57fa\u7840\u3001\u6700\u4e3b\u8981\u7684\u521b\u9020\u6d3b\u52a8\u662f\u9020\u7269\u3002<\/p>\n<h2>\u516b\u3001restful \u8bbe\u8ba1\u539f\u5219\uff1f<\/h2>\n<p>RESTful\u8bbe\u8ba1\u539f\u5219\uff08\u4e0d\u540c\u516c\u53f8\u5177\u4f53\u7ec6\u8282\u53ef\u80fd\u4e0d\u540c\uff09\uff1a<\/p>\n<p>\u5728\u63a5\u53e3\u547d\u540d\u65f6\u5e94\u8be5\u7528\u540d\u8bcd\uff0c\u4e0d\u5e94\u8be5\u7528\u52a8\u8bcd\uff0c\u56e0\u4e3a\u901a\u8fc7\u63a5\u53e3\u64cd\u4f5c\u5230\u662f\u8d44\u6e90\u3002<\/p>\n<p>\u5728url\u4e2d\u52a0\u5165\u7248\u672c\u53f7\uff0c\u5229\u4e8e\u7248\u672c\u8fed\u4ee3\u7ba1\u7406\u66f4\u52a0\u76f4\u89c2<\/p>\n<p>https:\/\/www.rgc.com\/v1\/<\/p>\n<p>\u5bf9\u4e8e\u8d44\u6e90\u7684\u64cd\u4f5c\u7c7b\u578b\u5e94\u8be5\u662f\u901a\u8fc7http\u52a8\u8bcd\u8868\u793a\u3002<\/p>\n<p>GET \/zoos\uff1a\u5217\u51fa\u6240\u6709\u52a8\u7269\u56ed<\/p>\n<p>POST \/zoos\uff1a\u65b0\u5efa\u4e00\u4e2a\u52a8\u7269\u56ed<\/p>\n<p>GET \/zoos\/ID\uff1a\u83b7\u53d6\u67d0\u4e2a\u6307\u5b9a\u52a8\u7269\u56ed\u7684\u4fe1\u606f<\/p>\n<p>PUT 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